Proteins & Enzymes, by Ellis Bell
Descriptions of basic theory and applications of various protein focused experimental techniques with real experimental examples
Chapter 1: Basic Concepts of Protein Purification and Characterization
Chapter 2: Protein Purification: Classical Approaches- part 1
Chapter 2: Protein Purification: Classical Approaches - part 2
Chapter 3: Protein Purification: Affinity Chromatography
Chapter 4: Molecular Weight Determination
Chapter 5: Determination of the Primary Structure of Proteins and Peptides
Chapter 6: Peptide Synthesis
Chapter 7: Chemical Modification: Sidechain Specific Reagents
Chapter 8: Chemical Modification: Affinity Reagents
Chapter 9: Secondary Structure of Proteins
CD Spectroscopy
Chapter 10: Tertiary Structure of Proteins
Chapter 11: Quaternary Structure
Chapter 12: Conformational Changes
Chapter 13: Enzyme Kinetics: a Review
Chapter 14: Two Substrate Kinetics
Chapter 15: Three Substrate Kinetics and Related Topics
Chapter 16: Deviations from Linear Kinetics
Chapter 17: Ligand Binding Studies
Chapter 18: Rapid Kinetics
Chapter 19: Use of Isotopes in Enzyme Kinetic Mechanism Analysis
Chapter 20: Effects of Temperature, pH and Isotopically Labeled Substrates on Enzyme Activity
Chapter 21: Allosteric Models of Enzyme Regulation