Wildtype and available Watermelon Glyoxysomal Malate Dehydrogenase mutants are in pQE vectors in E coli cells suitable for either DNA production or Protein Expression, are Ampicillin Resistant, and can be supplied as streaks on Agar Plates. Contact Jessica Bell, jessicabell@sandiego to request any of these biologics.
This resource lists the sequence of Watermelon Glyoxysomal Malate Dehydrogenase with available mutants in bold. For each available mutant, its association with one of six project areas [Active Site (Catalysis), Mobile Loop (Substrate Specificity etc), Interface (Allosteric Regulation), Citrate Regulation, Loops & Turns (Protein Folding & Stability) and Rossmann Fold (Cofactor Binding & Specificity)] is indicated, as are residues which have not to date been mutated. Full details of the project areas are listed under the appropriate section in the CURE Project Area tab. The project descriptions often also describe the equivalent residues in other species/isoforms allowing evolutionary connections to be made.
Ref indicates that prior work on that residues mutation has been published as a FASEB Journal abstract and the published abstrcat is linked to Ref and should be cited in any work derived from acquiring this mutant. The wildtype construct is described in Cox et al and should also be cited and is linked here
Linked to this table are .pse files that can be downloaded and opened with PyMol for each of these project areas illustrating the main areas of the protein involved in the project. An Excel File containing all the information from the table (no links to sequence or .pse files). This Excel file also contains information about which classes/faculty in the consortium are working with the various mutants/projects and can also be downloaded. This allows you to make collaborations with other faculty/Institutions to further the impact of both your CURE and the research. If you start work on residues not listed as mutants please send that information to Ellis Bell (jbell@sandiego.edu), and, as available, supply the appropriate DNA sequence file to be added to the table.
Each mutant is linked to its DNA sequence data file which can be downloaded and opened using Bio-Edit. Instructions for obtaining and using Bio-Edit to compare the mutant sequence data with the wild type data are linked here.
Watermelon Glyoxysomal Malate Dehydrogenase wildtype sequence data file
For ease of visualization in the following table, lines of normal and italic text are alternated
wgMDH mutant Active Site Mobile Loop Interface Citrate Regulation Loops & Turns Rossmann Fold
G44
F45 Rossmann beta1
K46 Rossmann beta1
V47 Rossmann beta1
A48 Rossmann beta1
I49 Rossmann beta1
L50 Loop Rossmann beta1
G51A Loop
A52S Loop
A53 Loop
G54 Loop
G55 Loop
I56 Rossmann alpha1
G57 Rossmann alpha1
Q58L Interface Region 1 Rossmann alpha1
P59 Interface Region1 Rossmann alpha1
L60 Rossmann alpha1
A61 Rossmann a1
Ref: M62 Interface Region1 Rossmann a1,
L63 Rossmann a1
Ref: M64L Interface Region1 Rossmann a1
K65 Interface Region 1-negative Rossmann a1
M66L Interface Region 1 Rossmann alpha1
N67 Turn Rossmann alpha1
P68 Turn
L69 Turn
V70 Turn Rossmann beta2
S71 Rossmann beta2
V72 Rossmann beta2
L73 Rossmann beta2
H74 Rossmann beta2
L75 Rossmann beta2
Y76L Rossmann beta2
Y76N Rossmann beta2
D77E Turn Rossmann beta2
D77E / V79P Turn Rossman beta2
V78 Turn
V79 Turn
N80 Interface Region 2 Turn Rossmann alpha3,
A81 Rossmann alpha3
P82 Interface Region 2 Rossmann alpha3
G83A Interface Region 2 Rossmann alpha3
V84 Interface Region 2 Rossmann alpha3
T85 Rossmann alpha3
A86 Interface Region 2 Rossmannn alpha3
Ref: D87L Interface Region 2 Rossmann alpha3
Ref: I88A Interface Region 2 Rossmann alpha3
S89 Rossmann alpha3
H90Q Interface Region 2 Loop Rossmann alpha3
Ref: H90R Interface Region 2 Loop Rossmann alpha3
Ref: M91Q Interface Region 2 Loop
D92 Interface Region 2 Loop
T93 Interface Region 2 Loop
G94 Interface Region 2 Loop
A95 Interface Region 2 Loop
V96 Loop Rossmann beta3
V97 Rossmann beta3
R98 Rossman beta3
G99 Rossmann beta3
F100 Rossmann beta3
L101 Rossmann beta3
G102 alpha
Q103 alpha
Q104 alpha
Q105 alpha
L106 alpha
E107 alpha
A108 alpha
A109 alpha
Ref: L110 alpha
T111 Turn alpha
G112 Turn
M113 Turn
D114 Turn
L115 Turn Rossmann beta4
I116 Rossmann beta4
I117 Loop Rossmann beta4
V118A Loop Loop Rossmann beta4
V118Q Loop Loop
P119W Loop Loop
Ref: A120G Loop Loop
Ref: A120V Loop Loop
Ref: A120W Loop Loop
Ref: G121A Loop Loop
Ref: G121F Loop Loop
Ref: G121I Loop Loop
V122K Loop Loop
V122L Loop Loop
Ref: P123A Loop Loop
Ref: P123F Loop Loop
Ref: P123G Loop Loop
Ref: P123S Loop Loop
Ref: R124A Active Site Loop Loop
R124A / R130A Active Site Loop Loop
R124E / M128L Active Site Loop Loop
Ref: R124K Active Site Loop Loop
Ref: R124L Active Site Loop Loop
Ref: R124Q Active Site Loop Loop
K125A Loop Loop
K125Q Loop Loop
K125R Loop Loop
P126E Loop Loop
P126G Loop Loop
P126H Loop Loop
P126R Loop Loop
P126W Loop Loop
G127A Loop Loop
G127F Loop Loop
G127P Loop Loop
G127V Loop Loop
Ref: M128A Loop Loop
Ref: M128I Loop Loop
Ref: M128Q Loop Loop
T129ins4A Loop Loop
T129E Loop Loop
T129V Loop Loop
Ref: R130A Active Site Loop Loop
Ref: R130E Active Site Loop Loop
Ref: R130Q Active Site Loop Loop
Ref: R130S Active Site Loop Loop
D131E Loop Helix 1 Rossmann alpha3
D131L Loop Helix 1 Rossmann alpha3
D131N Loop Helix 1 Rossmann alpha3
D132A Loop Helix 1 Rossmann alpha3
D132E Loop Helix 1 Rossmann alpha3
D132H Loop Helix 1 Rossmann alpha3
D132N Loop Helix 1 Rossmann alpha3
Ref: L133N Loop Helix 1 Bend Rossmann alpha3
Ref: L133S Loop Helix 1 Bend Rossmann alpha3
Ref: L133T Loop Helix 1 Bend Rossmann alpha3
Ref: L133V Loop Helix 1 Bend Rossmann alpha3
Ref: F134S Loop Helix 1 Bend Rossmann alpha3
K135 Loop Helix 1 Bend Rossmann alpha3
I136L Loop Helix 1 Bend Rossmann alpha3
I136W Loop Helix 1 Bend Rossmann alpha3
Ref: N137A Loop Helix 1 Bend Rossmann alpha3
Ref: N137D Loop Helix 1 Bend Rossmann alpha3
Ref: N137M Loop Helix 1 Rossmann alpha3
Ref: N137Q Loop Helix 1 Rossmann alpha3
A138 Loop Helix 1 Rossmann alpha3
G139 Loop Helix 1 Rossmann alpha3
I140E Loop Helix 1 Rossmann alpha3
V141 Helix 1 Rossmann alpha3
K142 Helix 1 Rossmann alpha3
T143 Helix 1 Rossmann alpha3
L144 Helix 1 Rossmann alpha3
C145 Helix 1 Rossmann alpha3
E146 Helix 1 Rossmann alpha3
G147 Helix 1 Rossmann alpha3
I148 Helix 1 Rossmann alpha3
A149 Helix 1 Rossmann alpha3
K150E Helix 1 Rossmann alpha3
C151 Helix 1 Turn
C152 Helix 1 Turn
P153 Helix 1 Turn
R154 Turn
A155 Turn
I156 Rossmann beta5
V157 Rossmann beta5
N158 Rossmann beta5
L159V Rossmann beta5
I160 Turn
S161 Turn
N162E Turn Rossmann alpha4
N162H Turn Rossman alpha4
P163 Turn Rossmann alpha4
V164 Rossmann alpha4
Ref: N165 Rossmann alpha4
Ref: S166 Rossmann alpha4
T167 Rossmann alpha4
V168 Rossmann alpha4
P169 Rossmann alpha4
I170 Rossmann alpha4
A171 Rossmann alpha4
A172 Rossmann alpha4
E173 Rossmann alpha4
V174 Rossmann alpha4
F175 Rossmann alpha4
K176 Rossmann alpha4
K177 Rossmann alpha4
A178 Rossmann alpha4
G179 Loop Rossmann alpha4
T180 Loop
Y181 Loop
D182 Loop
P183 Loop
K184 Loop
R185Q Loop
L186 Loop Rossmann beta6
L187 Turn Rossmann beta6
G188 Turn Rossmann beta6
Ref: V189W Turn
T190
M191
L192 Interface Region 3
Ref: D193E Active Site
Ref D193N Active Site
Ref: D193R Active Site
V194E Interface Region 3
V195 Interface Region 3
Ref: R196A Active Site Interface Region 3
Ref: R196E Active Site Interface Region 3
Ref: R196L Active Site Interface Region 3
Ref: R196M Active Site Interface Region 3
A197
N198 Interface Region 3
T199 Interface Region 3
F200
V201
A202
E203 Interface Region 3-negaive
V204
L205
G206 Loop
L207 Loop
D208 Interface Region 3 Loop
P209 Interface Region 3 Loop
R210A Interface Region 3 Loop
D211 Loop
V212 Interface Region 3 Loop
D213 Loop
V214 Loop
P215 Loop
V216T / T224V
V217
G218W Active Site Turn
G219A Active Site Turn
G219S Active Site Turn
Ref: H220 Active Site Turn
A221T Active Site
G222 Turn
V223 Turn
T224V Turn
I225 Turn
L226
P227 Loop
L228 Loop
Ref: L229 Loop
S230 Loop
Q231 Loop
V232 Loop
K233 Loop
P234 Loop
P235 Loop
S236 Loop
S237 Loop
F238 Loop
T239 Helix 2
Ref: Q240 Helix 2
E241 Helix 2
E242 Helix 2
I243 Helix 2
S244 Helix 2
Y245A Helix 2
L246 Helix 2
T247 Helix 2
D248 Helix 2
R249 Interface Region 4 Helix 2
I250V Helix 2 Bend
Q251A Helix 2 Bend
N252 Helix 2 Bend
G253 Interface Region 4 Helix 2 Bend
G254 Helix 2
Ref: T255E Interface Region 4 Helix 2
Ref: T255V Interface Region 4 Helix 2
Ref: E256Q Interface Region 4 Helix 2
V257 Interface Region 4 Helix 2
V258 Helix 2
E259 Helix 2
A260 Interface Region 4 Helix 2
Ref: K261M Interface Region 4 Helix 2
Ref: K261Q Interface Region 4 Helix 2
A262 Loop
G263 Loop
A264 Loop
G265 Loop
Ref: S266A Interface Region 4 Loop
A267 Interface Region 4 Loop
T268 Interface Region 4
Ref: L269A Interface Region 4
Ref: L269Q Interface Region 4
Ref: S270A Interface Region 4
Ref: S270C Interface Region 4
M271Q Interface Region 4
A272 Interface Region 4
Y273 Interface Region 4
A274
A275
V276 Interface Region 4
K277 Interface Region 4
F278
A279
D280
A281
C282
L283
R284
G285
L286
R287
G288 Turn
D289 Turn
A290E Turn
A290F Turn
G291 Turn
V292 Turn
I293
E294
C295
A296
F297
V298
S299 Loop
S300 Loop
Ref: Q301 Loop
V302 Loop
T303 Loop
E304 Loop
L305 Loop
P306 Loop
F307 Loop
F308
A309
S310
V313
R313
L314
G315 Turn
R316 Turn
N317 Turn
G318 Turn
Ref: I319W
E320
E321
V322 Loop
Y323 Loop
S324 Loop
L325 Loop
G326 Loop
P327 Loop
L328 Loop
N329 Loop
E330
Y331
E332
R333
I334
G335
L336
E337
K338
A339
K340
K341
E342
L343
A344
G345
S346
I347
E348
K349
G350
V351
S352
F353C
I354
R355
S356